SSC Buffer 20× Concentrate for Northern and Southern blotting, solution

Stock code :S6639
Brand :Sigma-Aldrich
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SSC Buffer 20× Concentrate for Northern and Southern blotting, solution

General description

SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization.

Application

SSC Buffer 20× Concentrate has been used
• as a hybridization buffer on in situ hybridization human-specific repetitive Alu sequence[14]
• as a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA[2] and northern blotting of microRNA[3]
• as an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon[4]

Features and Benefits

• Convenient ready-to-use liquid
• Multi-purpose use in hybridizations

Components

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

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