pBR322 Hae III Digest
Sigma’s pBR322 Hae III Digest contains 22 fragments from 8 - 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Sigma’s pBR322 Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
Contains 22 fragments, 8–587 bp
For optimal resolution, the recommended agarose gel concentration is 2.5.
Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction.
