PCR Buffer Without MgCl2, 10x concentrated
The PCR buffer without MgCl2, in combination with the MgCl2 stock solution, is used for the individual adjustment of the Mg2+ concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl2 will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg2+ concentration to increase the specificity and yield of the PCR reaction.
The PCR buffer without MgCl2 is used for the amplification of specific DNA fragments by the polymerase chain reaction (PCR).[1][2]
The buffer is ten-fold concentrated and
should be used in combination with the MgCl2 Stock Solution.
Solution, 100 mM Tris-HCl, 500 mM KCl (pH 8.3 at 20 °C)
For general laboratory use.
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