Single-strand Binding Protein from Escherichia coli >95% (SDS-PAGE)
Single-strand Binding Protein is a homotetramer,[2] found in E. coli during the event of DNA metabolism.[1]
Binds with high specificity to single-stranded DNA. Can be useful in enhancing the specificity of PCR and in enabling the sequencing of problematic DNA templates.
Single-strand Binding Protein from Escherichia coli has been used in in vitro RNA/Single-Strand Binding Protein (SSB) complex formation assays[4] and in protein purification to determine integrity and functionality of the integron attC sites.[5]
Single-strand Binding Protein (SSBP) exhibits high affinity towards single stranded DNA important for DNA metabolic processes.[2][3] Single-stranded DNA-binding protein hSSB1 is critical for genomic stability and assists the delivery of primers during the formation of lagging standard.[3] It also prevents the formation of inhibitory secondary structures in the exposed lagging-strand of DNA during replication.[3] SSBP mediates the recruitment of other proteins to their sites of function on DNA.[2]
Solution in 20 mM Tris-HCl, pH 8.0, 0.5 M NaCl, 0.1 mM EDTA, 0.1 mM DTT, 50% glycerol
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