M-MLV Reverse Transcriptase Moloney Murine Leukemia Virus enzyme & buffer
M-MLV (Moloney murine leukemia virus) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues.[5] It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.
M-MLV Reverse Transcriptase has been used:
• to synthesize cDNA[1]
• in quantitative realtime-polymerase chain reaction (RT-qPCR)[2]
• in reverse transcription[3]
M-MLV Reverse Transcriptase has been used:
• for the preparation of cDNA libraries or for first strand cDNA synthesis[6]
• for use in a 2-step RT-PCR assay[4][7][10]
• for
the synthesis of cDNA that is further used in cloning[8]
M-MLV (Moloney Murine Leukemia Virus) reverse transcriptase is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand. M-MLV is used for the preparation of cDNA libraries or for first strand cDNA synthesis for use in RT-PCR reactions.
• Thermostable reverse transcriptase active at 37 °C.
• Can be used to generate first strand cDNA of up to 7 kb.
Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.
