Primer for cDNA Synthesis
This primer consists of a 15-mer oligonucleotide that shows thymidine (T) at each individual position. The oligonucleotides are HPLC purified, desalted, and supplied as a lyophilizate of the Li-salt. The p(dT)15 primer is
not phosphorylated at the 5′ end.Oligo(dT)N generates full-length cDNAs. If template contains oligo(A) stretches internally, the primer may bind these, causing mis priming and transcripts that are not full-length.
Composition: p(dT)15[ 15-mer oligonucleotide containing only deoxythymidine (dT) residues].
Phosphorylation: The p(dT)15 primer is not phosphorylated at the 5′ end.
Primer for cDNA synthesis may be used in:
• RT-PCR[1][2][3]
• Generation of cDNA libraries
• Synthesis of first-strand
cDNA
8 nmol ξ 1 A260 unit
For life science research only. Not for use in diagnostic procedures.
