SYBR® Green Quantitative RT-qPCR Kit One step SYBR® Green RT-qPCR
The SYBR® Green Quantitative RT-PCR Kit combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT), JumpStart™ Taq DNA polymerase, and SYBR Green I fluorescent dye in a one-step RT-PCR kit designed for measurement of gene expression. This convenient 2X ready mix includes M-MLV RT, SYBR Green I dye, JumpStart™ Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.
SYBR® Green Quantitative RT-qPCR Kit has been used in a 1-step reverse transcription polymerase chain reaction (RT-PCR) assay to detect specific genetic clusters of genogroup I and II noroviruses[1], for chikungunya viral (CHIKV) RNA quantification[2], and to detect mRNA expression levels.[3][5]
The SYBR Green Quantitative RT-PCR kit provides a highly sensitive method for the quantitative analysis of gene expression. Sigma′s QRT-PCR ReadyMix combines the advantages of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart Taq in a convenient ReadyMix.
• The master mix allows consistency and reproducibility from one reaction to the next
• Reduced preparation time and the risk of contamination from multiple pipetting steps
• Reduced set-up time as compared to manual or wax Hot
Start methods
• JumpStart™Taq Polymerase reduces primer-dimer and non-specific product formation
• SYBR® Green I dye is inexpensive, easy to use, and highly sensitive
• Broad instrument compatibility
• Includes a separate ROX reference dye vial for reaction normalization
1 kit sufficient for 100 reactions at 50 μL each
