Viral RNA Extraction Buffer One Step RT-qPCR Compatible, Optimized
The Viral RNA Extraction Buffer has been optimized to provide rapid, room-temperature lysis of SARS-CoV-2 viral particles in saliva. The buffer comes as a 4x working solution that can be mixed with virus-containing samples in a qRT-PCR plate. Upon 5 minute incubation at room temperature, viral particles are lysed and RNA is stabilized such that samples are ready for direct analysis via RT-qPCR with no additional cleanup step. The Viral RNA Extraction Buffer is compatible with several one-step RT-qPCR kits, including Sigma’s Quantitative RT-PCR ReadyMix (QR0200). Removal of precipitates in saliva via centrifugation prior to testing is recommended for optimal sensitivity.
The Viral RNA Extraction Buffer is broadly compatible with most nucleic acid detection methods and does not reduce signal when used at working concentration in qRT-PCR. qRT-PCR compatibility has been demonstrated in a 4-fluor multiplex setting consisting
of Cy5, TxRed, FAM, and HEX.
RT-qPCR compatibility has been verified with several one-step RT-qPCR kits:
• Quantitative RT-PCR ReadyMix™ (Sigma-Aldrich, catalog QR0200)
• KAPA PROBE FAST One-Step Universal (Sigma-Aldrich,
catalog KK4752)
• KiCqStart® One-Step Probe RT-qPCR ReadyMix™ (Sigma-Aldrich, catalog KCQS07)
• TaqPathTM 1-Step Multiplex Master Mix (Thermo Fisher, catalog A28525)
The Viral RNA Extraction Buffer is also
compatible with colorimetric LAMP assays.
• Extract viral RNA without cleanup
• Directly compatible with qRT-PCR and LAMP assays
• Compatible with saliva, saline, Amies medium, and viral transport medium
• More sensitive than other direct saliva-based RNA detection
methods
KiCqStart is a registered trademark of Qiagen Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
