BFA
Bovine aorta endothelium foetal
Derived from a bovine foetus.
Virus studies
Ham′s F12 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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