Merck Thailand

4′,6-Diamidino-2-phenylindole dihydrochloride powder, BioReagent, suitable

for cell culture, ≥98% (HPLC and TLC), suitable for fluorescence

Stock code :D8417

Brand :Sigma-Aldrich

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4′,6-Diamidino-2-phenylindole dihydrochloride powder, BioReagent, suitable

General description

4′,6-Diamidino-2-phenylindole dihydrochloride is a fluorescent dye for staining DNA.^[1] It strongly interacts with the dAT-rich (deoxy adenine-thymine) regions of the DNA.^[2]

Application

4′,6-Diamidino-2-phenylindole dihydrochloride has been used in staining of various cells in immunocytochemistry and cytochemistry, ^[3] human subcutaneous adipose tissues for fluorescence imaging,^[4] embryo sections for immunofluorescence.^[5]

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Packaging

1, 5, 10 mg in glass bottle

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