Human Laminin (pepsinized) Purified Protein
| Human Laminin (pepsinized) Purified Protein |
| Overview |
AG56P contains a mixture of human laminins containing the beta1 chain, principally laminin 1, 2, 3, 6, 8 & 10. This preparation is immunologically and biologically identical to intact human laminin. AG56P is suitable for use in ELISA, production
of antiserum, cell adhesion and attachment assays, and neurite-stimulation assays. This pepsinized laminin preparation runs as twin bands migrating at approximately 160kDa and 130kDa by SDS-PAGE under reducing conditions.
Preparation:
Chemicon's purified human laminin is prepared from freshly frozen human placenta tissue that is homogenized in PBS with a blender and the mixture centrifuged. The pellet is then washed with 0.5M acetic acid, collected and subjected to and extended
Pepsin A digestion. The pepsin digest is neutralized, centrifuged to clear debris, and applied to an antibody column containing mouse anti-human laminin monoclonal 4E10 monoclonal antibody {MAB1921}. The solubilized laminin solution is passed
over the column, and utlimately the laminin is released via acidification with KSCN. Then the material is dialyzed against PBS. The antibody 4E10 is specific for laminin beta1 chain. This chain is found in Laminin's 1,2,3,6,8,10 under the native
conditions used to purify the material. |
More detail
