SMC Human Interleukin 6 (IL-6) High Sensitivity Immunoassay Kit
Interleukin 6 (IL-6) is secreted by T cells and macrophages and acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Some of its functions include the stimulation of the immune response during infection, induction of fever in autoimmune,
infectious and non-infectious diseases, the stimulation of acute phase reactions, differentiation of T cells, proliferation and differentiation of B-cells, the development of blood cells as well as the activation of osteoclasts and osteoporosis and
the secretion of vascular endothelium growth factor (VEFG) to increase blood vessel growth and vascular permeability in inflammation. It is important for a short-term defense against infection, injury and inflammation however dysregulation of IL-6
results in disease.
IL-6 is important in the study of diseases such as diabetes, lupus, multiple myeloma, rheumatoid arthritis and some cancers such as prostate and pancreatic cancer. Conversely, deficiency of IL-6 can have an effect on
immune activation and IgA antibodies while overexpression of IL-6 can result in an immunological imbalance leading to an autoimmune pathology.
The SMC™ Human IL-6 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to
existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna
Although IL-6 was detectable in non-human primate (rhesus macaque and cynomolgus monkey) samples, the assay has not been validated for these species.
Research Category
Inflammation & Immunology
Research Sub Category
Inflammatory Disease
Immune Response
Autoimmune disease
The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC
This new streamline kit has been improved with the following features while the kit performance is consistent with the legacy IL-6 kit, #03-0089-01:
• Improved options for sample prep and incubation
• Lyophilization of the standard provides
the kit at one storage condition
• Overnight incubation can be used
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO™ and Erenna
Assay Buffer
Human IL-6 Beads
Standard Diluent
Human IL-6 Detection Antibody
Human IL-6 Standard
10X Wash Buffer
Buffer D
Elution Buffer B
Minimum of 5 samples spiked with average recovery of 107% with a range of 90-114%.
SMC is a trademark of Singulex
SMCxPRO is a trademark of Merck KGaA, Darmstadt, Germany
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
