SMC™ Human Vascular Endothelial Growth Factor
The SMC™ Human VEGF Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
VEGF-A, commonly known as VEGF, is one member of a family of secreted glycoproteins that promote endothelial cell growth, survival, migration, and vascular permeability, all of which contribute to angiogenesis. The binding of VEGF to its receptor triggers the activation of a cell signaling pathway that is critical for the growth of blood vessels from pre-existing vasculature. VEGF is implicated in a variety of diseases, including several cancers, age-related macular degeneration, diabetic retinopathy and rheumatoid arthritis. With the efficacy seen with drugs that target VEGF and its pathway, VEGF will remain an attractive candidate for the development of therapies for these diseases, particularly cancer.
Research Category
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
The sensitivity of Single Molecule Counting (SMC™) gives the SMC™ Human VEGF Immunoassay Kit unparalleled performance in the quantification of VEGF in human EDTA plasma.
VEGF Coated Beads
Standard Diluent
VEGF Detection Antibody
Assay Buffer
VEGF Standard
SMC™ Human VEGF Immunoassay Kit Instructions
10X Wash Buffer
Elution Buffer B
Buffer D
Low-end CV% Range: 2 - 15%|Low-end CV% Average: 7%
SMC is a trademark of Singulex
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
