Fast Green FCF Dye content ≥85 %

Stock code :F7252
Brand :Sigma-Aldrich
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Fast Green FCF Dye content ≥85 %

General description

Fast Green FCF is one of the triarylmethane dyes,[1] that is biocompatible.[2] This triphenylmethane color additive is mainly used in coloring food, drugs and cosmetics. [2]

Application

Fast Green FCF has been used for in staining of menisci from rabbit[3] and of osteochondral tissues.[4]

Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

Packaging

5, 25, 100 g in glass bottle

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